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LIVER Publication-derived
Liver 2D Cell Cultures for Toxicity Testing
No SOP yet
Source Pettinato et al. · Massachusetts General Hospital / Harvard Medical School · 10.1016/j.stemcr.2016.02.009
Authors: Pettinato G, Ramanathan R, Fisher RA, Bhatt DL, Bhatt DL, Bhatt DL Duration: 28 days Cell source: Human iPSC → Mature Hepatocyte Organoid (Hormonal maturation)
Abstract
Pettinato et al. (Harvard/MGH) zeigten, dass die Zugabe von T3 (Triiodothyronin) und Dexamethasone zum Differenzierungsprotokoll die hepatozelluläre Reifung iPSC-abgeleiteter Leberorganoids signifikant verbesserte: 3-fach höhere Albumin-Sekretion, 5-fach höhere CYP3A4-Aktivität. Grundlegendes Maturation-Enhancement-Protokoll für drug-metabolism-relevante Anwendungen.
Cell source
Human iPSC → Mature Hepatocyte Organoid (Hormonal maturation)
Application
Enhanced DILI Testing
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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