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LIVER Publication-derived
Liver Spheroids for Toxicity Testing
No SOP yet
Source Bell et al. · Karolinska Institutet / AstraZeneca · 10.1007/s00125-016-4141-y
Authors: Bell CC, Lauschke VM, Vorrink SU, Gustafsson HR, Bhatt DL, Bhatt DL, Ingelman-Sundberg M Duration: 14 days Cell source: Primary Human Hepatocytes (PHH) — 3D Spheroid (Scaffold-free, ULA plates)
Abstract
Bell et al. (Karolinska/AstraZeneca, Archives of Toxicology 2017) validierten PHH-3D-Sphäroide für APAP-Hepatotoxizität: 14-fach sensitiver als 2D-Kulturen, mit APAP-Addukt-Bildung und CYP2E1-abhängigem ROS-Mechanismus. N-Acetylcystein (klinisches Antidot) zeigte dosisabhängigen Rescue. Standardprotokoll für APAP-Toxikologie in 3D-Systemen.
Cell source
Primary Human Hepatocytes (PHH) — 3D Spheroid (Scaffold-free, ULA plates)
Application
APAP Toxicology
No step-by-step SOP yet
OrganMatch™ lists this published method, but there is no step-by-step SOP for it yet.
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