LIVER Publication-derived

Acetaminophen (APAP) Acute Liver Injury Model in Liver Organoids

Source Bell et al. · Karolinska Institutet / AstraZeneca · 10.1007/s00125-016-4141-y

👤 Bell CC, Lauschke VM, Vorrink SU, Gustafsson HR, Bhatt DL, Bhatt DL, Ingelman-Sundberg M ⏱ 6720 min 🧫 Primary Human Hepatocytes (PHH) — 3D Spheroid (Scaffold-free, ULA plates)

Abstract

Bell et al. (Karolinska/AstraZeneca, Archives of Toxicology 2017) validierten PHH-3D-Sphäroide für APAP-Hepatotoxizität: 14-fach sensitiver als 2D-Kulturen, mit APAP-Addukt-Bildung und CYP2E1-abhängigem ROS-Mechanismus. N-Acetylcystein (klinisches Antidot) zeigte dosisabhängigen Rescue. Standardprotokoll für APAP-Toxikologie in 3D-Systemen.

Cell source

Primary Human Hepatocytes (PHH) — 3D Spheroid (Scaffold-free, ULA plates)

Application

APAP Toxicology

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in Bell et al.. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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