Cortical 2D Cell Cultures for Disease Modelling
Source Mariani et al., 2012 · Yale University School of Medicine · 10.1073/pnas.1202944109
Abstract
This protocol generates three-dimensional multilayered structures from human induced pluripotent stem cells (hiPSCs) that recapitulate early dorsal telencephalic development in vitro. The structures contain polarized radial glia, intermediate neuronal progenitors, and layer-specific cortical neurons with excitatory and inhibitory phenotypes, mimicking the organization of the developing human cerebral cortex at 8-10 weeks postconception.
Protocol overview
9 steps across 4 phases
- a Culture undifferentiated hiPSC lines
- b Dissociate hiPSC colonies into single cells
- a Initiate suspension culture with neural induction factors
- b Monitor aggregate formation
- a Transfer aggregates to coated surface
- b Maintain differentiation without additional growth factors
- c Allow neuronal maturation and layer organization
- a Continue culture to day 70 for synaptic maturation
- b Monitor astroglial differentiation
Full SOP
Full SOPs need an account and a plan
Create an account, then get free Academic access (for verified academics) or choose a plan. Plans include step-by-step procedures, reagent concentrations, QC checklists and batch record templates.
Create an accountAlready registered? Log in
Attribution
This SOP was written by Organthis based on the published method in Mariani et al., 2012. Once the originating laboratory has confirmed it, the SOP is marked Verified.
Something wrong with this entry? Report an issue with this protocol