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PANCREAS Publication-derived

Multi-center PDAC

Source Utrecht/MSKCC/MD Anderson

⏱ 60 days 📋 4 phases 🧫 Patient tumor

Abstract

Cell source
Patient tumor
Application
PDAC modeling

Protocol overview

12 steps across 4 phases

Tissue Acquisition and Processing Day 0 (within 4 h of collection)
  1. 1 Receive and document surgical or biopsy specimen
  2. 2 Mechanical dissection
  3. 3 Collagenase II digestion
  4. 4 Sequential straining, washing, and red cell lysis
  5. 5 Count viability and assess sample quality
Matrigel Dome Plating Day 0
  1. 6 Prepare Matrigel domes with PDAC cells
  2. 7 Plate domes and polymerise
  3. 8 Overlay with POM + ROCK inhibitor
Culture Monitoring and Establishment Day 1–14
  1. 9 Monitor daily and refresh medium
Passage, Expansion, and Cryopreservation Day 7–14 (first passage); then every 7–14 days
  1. 10 Recover organoids from Matrigel
  2. 11 Fragment organoids for passage
  3. 12 Re-embed in Matrigel and cryopreserve

Full SOP

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Attribution

This SOP was authored by Organthis based on the published method in the published method. The originating laboratory holds no rights in this SOP and has not endorsed it unless marked Verified.

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