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BRAIN Publication-derived

Schizophrenia Cerebral Organoids (2022)

Full SOP with Academic or a plan

Source Notaras et al., 2022 · Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, Cornell University · 10.1038/s41380-021-01316-6

Authors: Michael Notaras, Aiman Lodhi, Friederike Dündar, Paul Collier, Nicole M. Sayles, Hagen Tilgner, David Greening, Dilek Colak Duration: 42 days 5 phases Cell source: Patient-Derived iPSC (Schizophrenia)

Abstract

This protocol generates 3D patient-derived cerebral organoids from idiopathic schizophrenia and healthy control iPSC lines to model cell-specific neuropathology during early brain development. The organoids recapitulate ventricular zone disruption, altered neurogenesis, and molecular signatures of schizophrenia, enabling mechanistic study of developmental disease mechanisms.

Cell source
Patient-Derived iPSC (Schizophrenia)
Application
Disease modeling of early neurodevelopmental pathology in Schizophrenia

Protocol overview

13 steps across 5 phases

Phase 1 · iPSC Maintenance and Quality Control Ongoing (before organoid generation)
  1. a Maintain iPSC lines on Vitronectin-coated plates
  2. b Screen control lines for mental health history
Phase 2 · Embryoid Body Formation Days 0-7
  1. a Dissociate iPSC colonies
  2. b Culture embryoid bodies in ultra-low attachment plates
  3. c Monitor embryoid body quality
Phase 3 · Neural Fate Specification and Neuroepithelium Formation Days 7-14
  1. a Transfer embryoid bodies to neural induction plates
  2. b Monitor for neuroepithelium formation
Phase 4 · Matrigel Embedding and 3D Expansion Days 14-21
  1. a Embed organoids in Matrigel droplets
  2. b Culture organoids without agitation
  3. c Initiate orbital shaker culture
Phase 5 · Extended Maturation and Maintenance Days 21 onwards (typically to day 35-42 for core phenotyping)
  1. a Continue orbital shaker culture with media changes
  2. b Rolling quality control assessment
  3. c Harvest organoids for experimentation

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Attribution

This SOP was written by Organthis based on the published method in Notaras et al., 2022. Once the originating laboratory has confirmed it, the SOP is marked Verified.

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